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testing #ATP to improve tissue growth and mechanical properties without the need for mechanical loading. They found that while high doses of ATP (250 μM) had a positive effect, it also caused a catabolic response, which is when the tissue breaks down. To find the optimal dose of ATP, the researchers tested different doses (31.25, 62.5, and 125 μM) to see which one had the best effect on tissue growth and mechanical properties without causing a catabolic response.
#Calcium is an important factor in the ATP-mediated catabolism process. The researchers found that when they added 10 μM PPi to #chondrocyte cultures, there was a 32% increase in MMP-13 activity compared to unstimulated controls. This effect appeared to require calcium and could be inhibited by the MEK1/2 inhibitor U0126.
Additionally, #TEM imaging was conducted on engineered cartilaginous tissues supplemented with 0, 62.5 and 250 μM ATP but no mineralization or CPPD crystals were observed which suggests that these doses of ATP did not cause any catabolic response due to crystal formation.
The text is discussing a method of improving tissue growth and mechanical properties of engineered #cartilage constructs by applying mechanical loading.
However, this approach has limitations when dealing with irregular geometry and high radii of curvature. An alternative approach is to use the known mechanotransduction pathways responsible to achieve the same effect without externally applied forces.