They used @wnt activator and #BMP inhibitors at the beginning of the differentiation process to induce paraxial #mesodermal @cell s. They then added #FGF2 to the Matrigel to promote the 3D structure. #HGF and IGF1 were added later to accelerate the #myogenic specification and further #myofiber differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed @neuralcell s and withdrew FGF2 to focus on muscle tissue development.