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Joseph P.

They used @wnt activator and inhibitors at the beginning of the differentiation process to induce paraxial @cell s. They then added to the Matrigel to promote the 3D structure. and IGF1 were added later to accelerate the specification and further differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed @neuralcell s and withdrew FGF2 to focus on muscle tissue development.