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This preprint looks like a conceptual advance for deeper staining (also in human tissue) and multi-round multiplexed immunostaining with BABB clearing.
INSIHGT: Accessible multimodal systems biology with quantitative molecular phenotyping in 3D
Yau et al., preprint at biorxiv 2024
https://doi.org/10.1101/2024.05.24.595771
![Fig. 2A: (A) Experimental steps and principle of INSIHGT for
immunostaining. Top row: Tissue is infiltrated with antibodies and a weakly coordinating superchaotrope ([B12H12]
2-, purple
dodecahedron) in the 1st staining solution and then transferred into the 2nd solution containing a complexation agent (CD, red
ring). Bottom row: The molecular principles of INSIHGT. Weakly coordinating anion prevents antibody-antigen interactions,
removing penetration obstacles. After homogeneous infiltration, subsequent γCD infiltration complexes the [B12H12]
2- ions,
allowing deep tissue immunostaining.](https://static.mstdn.science/media_attachments/files/112/631/740/394/622/517/original/f3d4468ddafa53da.png "Fig. 2A: (A) Experimental steps and principle of INSIHGT for
immunostaining. Top row: Tissue is infiltrated with antibodies and a weakly coordinating superchaotrope ([B12H12]
2-, purple
dodecahedron) in the 1st staining solution and then transferred into the 2nd solution containing a complexation agent (CD, red
ring). Bottom row: The molecular principles of INSIHGT. Weakly coordinating anion prevents antibody-antigen interactions,
removing penetration obstacles. After homogeneous infiltration, subsequent γCD infiltration complexes the [B12H12]
2- ions,
allowing deep tissue immunostaining.")
