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#Isolation and genetic #characterization of #MERS-CoV from dromedary #camels in the #UAE, Front Vet Sci.: https://doi.org/10.3389/fvets.2023.1182165 #research #enzootic #zoonoses #betacoronavirus #merbecovirus
FrontiersIsolation and genetic characterization of MERS-CoV from dromedary camels in the United Arab EmiratesBackgroundThe study of coronaviruses has grown significantly in recent years.Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in various cell types, and quick development has been made of assays for its growth and quantification. However, only a few viral isolates are now available for investigation with full characterization. The current study aimed to isolate MERS-CoV from nasal swabs of dromedary camels and molecularly analyze the virus in order to detect strain-specific mutations and ascertain lineage classification.MethodsWe isolated the virus in Vero cells and adapted it for in vitro cultivation. The isolates were subjected to complete genome sequencing using next-generation sequencing followed by phylogenetic, mutation, and recombination analysis of the sequences.ResultsA total of five viral isolates were obtained in Vero cells and adapted to in vitro cultures. Phylogenetic analysis classified all the isolates within clade B3. Four isolates clustered close to the MERS-CoV isolate camel/KFU-HKU-I/2017 (GenBank ID: MN758606.1) with nucleotide identity 99.90–99.91%. The later isolate clustered close to the MERS-CoV isolate Al-Hasa-SA2407/2016 (GenBank ID: MN654975.1) with a sequence identity of 99.86%. Furthermore, the isolates contained several amino acids substitutions in ORF1a (32), ORF1ab (25), S (2), ORF3 (4), ORF4b (4), M (3), ORF8b (1), and the N protein (1). The analysis further identified a recombination event in one of the reported sequences...
''Conclusion: Data presented in this study indicated need for continuous identification & characterization of MERS-CoV to monitor virus circulation in region, which is necessary to develop effective control measures. #Mutations described in this investigation might not accurately represent virus’s natural evolution as artificial mutations may develop during cell culture passage. Isolated MERS-CoV strains would be helpful in new live attenuated vaccine development and efficacy studies.''