GlycoShape<p>A spoonful of sugar #11 🍬</p><p>As the rest of the community, we are excited about <a href="https://mstdn.science/tags/AF3" class="mention hashtag" rel="tag">#<span>AF3</span></a> and have been testing its performance in the reconstruction of glycans and glycosylation. We have found the following limitations users should be aware of:</p><p>- The structure of the Asn-linkages is incorrect</p><p>- All N-glycans have an a2 arm, while the Man should be in a3</p><p>What we suggest 😜 is to build the protein with AF3 and to reglycosylate it with ReGlyco at <a href="https://glycoshape.org" target="_blank" rel="nofollow noopener noreferrer" translate="no"><span class="invisible">https://</span><span class="">glycoshape.org</span><span class="invisible"></span></a> :awesome: </p><p><a href="https://mstdn.science/tags/glycotime" class="mention hashtag" rel="tag">#<span>glycotime</span></a></p>