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Moritz Negwer

Wow, TRISCO (née TRIC-DISCO) is out in Science! This cool approach allows imaging of mRNA transcripts throughout the cleared mouse brain. It uses a signal amplification step with in situ Hybridization Chain Reaction (isHCR) and combines it with DISCO-style solvent-based to make the brain transparent.

Whole-brain spatial transcriptional analysis at cellular resolution
Kanatani et al., Science 2024
doi.org/10.1126/science.adn994

Fig. 3. Multiplexed TRISCO staining of whole brains. (A) Schematic of the TRISCO protocol spanning 15 days. (B) (Top) 3D rendering of the whole brain from an 8-week-old adult mouse stained using TRISCO for cortical interneuron transcripts: Somatostatin (Sst mRNA, red), Parvalbumin (Pvalb mRNA, green), and Glutamate decarboxylase 1 (Gad1 mRNA, blue). (Bottom) Single-channel views. Bounding box, 7.7 × 10.6 × 5.5 mm. (C) Optical cross section at z = 2875 μm of the TRISCO brain in (B). (Right) Single-channel views. (Bottom) Magnified views of the hippocampus region at the indicated box. Nuclear staining was done with DiYO-1. Scale bars, 1 mm (white) and 200 μm (yellow).
#neuroscience#lightsheet#microscopy
Nov 24, 2024, 03:31 PM·Public·Web
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