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Moritz Negwer

New uses a custom mouse behaviour + deep-learning c-Fos analysis pipeline to compare several different Serotonin receptor agonists / antagonists in mice.

Concerted modulation of spontaneous behavior and time-integrated whole-brain neuronal activity by serotonin receptors
Friedmann et al., preprint at biorxiv 2024
doi.org/10.1101/2024.08.02.606

Fig. 3 Overview of the effect of serotonin receptor agonists and antagonists on whole-brain Fos maps. a, Experimental design for whole-brain analysis pipeline of Fos image data. b, Left, horizontal view of a 500-µm Z-projection of raw Fos image data. Right, zoom in of the boxed region from the left image (top), which is segmented by 3D-Unet based TrailMap. Centroids are established as local maxima detected from the probability maps and a 6-connected neighborhood of raw intensity values is averaged to be the intensity of each centroid. c, Horizontal (left) and coronal (right) depth-coded views of a single brain’s detected centroids demonstrate even coverage across the entire intact brain. d, Total numbers of counted nuclei per mouse and grouped by drug treatment. e, Scatter plot of average Fos intensity against the total Fos count (as in d) for all 168 mice, color coded by drug treatment. Linear fit shows significant correlation, R2 = 0.281. f, A representative 100-µm coronal slice of saline-subtracted Fos intensity maps for each drug treatment group, averaged across individuals within treatment groups. g, Coronal slices (as in f) across anteroposterior axis for two example drug treatment groups. h, Average saline-subtracted Fos intensity for the two drug treatment groups in g for all nuclei in each of 282 Allen-brain regions at the bottom, color coded according to Allen Brain Atlas38. Each dot represents data from an individual mouse.
#lightsheet#microscopy#serotonin
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